Ukugcinwa kwemethylene blue, ukuphelelwa kwayo nexesha ehlala ilungele ukusetyenziswa

Imethylene blue ayinalo ixesha elinye lokuhlala ilungele ukusetyenziswa elisebenza kuzo zonke iimveliso. Umgubo otywiniweyo, isinyibiliko esilungiselelwe ngamanzi, kunye neyeza elitofwayo ledosi enye ziimveliso ezahlukeneyo. Imihla yazo ixhomekeke kwinto eyenziwe ngayo imveliso, indlela eyenziwe ngayo, ukupakishwa, iimeko zokugcina, kunye nobungqina obuxhasa ukuzinza kwayo.

Qala ngesikhongozeli sakho. Fumana inombolo yebhetshi yaso, imiyalelo yokugcina, nomhla wokuphelelwa okanye umhla ekucetyiswa ukuba isetyenziswe ngaphambi kwawo. Emva koko jonga ukuba kukho umyalelo owahlukileyo wokuvulwa okanye wokulungiswa. Intetho ekwi-intanethi efana nokuthi “imethylene blue ihlala iminyaka” ayinakuqinisekisa ukuba loo bhotile ithile ihlala ilungele ukusetyenziswa ixesha elingakanani.

Oko uchazwa ngumhla ngokwenene

Kumayeza, iFDA icacisa ukuba imihla yokuphelelwa ibonisa ukuzinza okuqinisekisiweyo phantsi kweemeko zokugcina ezichazwe kwileyibhile. Ukuzinza kuquka ukugcina amandla, umgangatho nobunyulu obufunekayo. Umhla unxulumene nobungqina ngemveliso, hayi nje negama lesithako sayo esiphambili.

Imihla eyahlukeneyo iphendula imibuzo eyahlukeneyo:

Umhla wokuveliswa okanye wokukhutshwa: xa kwenzeka isiganeko sokuvelisa okanye isigqibo sokukhupha ibhetshi. Oku akuwenzi ngokuzenzekelayo ube ngumhla wokugqibela wokusetyenziswa.
Umhla wokuphelelwa okanye wokugqibela wokusetyenziswa: ukuphela kwexesha elimiselweyo lemveliso echaziweyo phantsi kweemeko ezichazwe kuyo.
Umhla wokuphinda kuvavanywe: ixesha apho into kufuneka iphinde ihlolwe ngokufanelekileyo ngokuchasene neemfuno ezichaziweyo zayo. Ayiyomvume yokwandisa ixesha lokuyisebenzisa ngokujonga umbala wayo.
Umda emva kokuvulwa okanye kokulungiswa: imiyalelo esebenza xa iphakheji yokuqala ivuliwe okanye indlela esenziwe ngayo isinyibiliko itshintshiwe. Kwimveliso elungiselelwe yaza yanikezelwa ekhemesti, oku kunokuvela kwileyibhile yekhemesti njengomhla ekungafanele isetyenziswe emva kwawo.

Uxwebhu lukaMerck olumalunga nemihla yeemveliso lwahlula imihla yokukhutshwa, yokuphelelwa neyokuphinda kuvavanywe. Lo mahluko ubalulekile xa kufundwa isatifikethi sesithako. Umhla wokuphinda kuvavanywe kumgubo wemethylene blue awuqinisekisi umhla wokuphelelwa kwesinyibiliko esenziwe ngawo kamva. Kanti nomhla wemveliso engakavulwa awuphenduli ngokuzenzekelayo yonke imibuzo ngesikhongozeli esivuliweyo.

Ngaba imethylene blue kufuneka igcinwe efrijini?

Musa ukuyifaka efrijini ngokuzenzekelayo. Landela umyalelo wobushushu waloo mveliso ithile.

Isikhokelo sikaBlupreme sokugcina isinyibiliko sichaza ukuba masigcinwe sime nkqo, sikhuselwe ekukhanyeni, nakubushushu obungaphantsi kwe-25 °C. Sithi ukugcinwa efrijini akuyomfuneko kwaye isinyibiliko asifanele senziwe umkhenkce. Gcina ibhotile ivalwe ngokuqinileyo uze ulandele umhla ekucetyiswa ukuba isetyenziswe ngaphambi kwawo okwipakheji yayo. Icandelo lemibuzo ebuzwa rhoqo ngoku alichazi xesha lahlukileyo emva kokuvulwa; oko kungabikho kwalo akuniki bungqina benani leenyanga elitsha eliqanjiweyo.

Imveliso eyahlukileyo inika umzekelo oluncedo wokuthelekisa. Ileyibhile yeyeza elitofwayo iPROVAYBLUE ichaza i-20–25 °C, ivumela nokuphambuka okwethutyana ukuya kwi-15–30 °C. Iyakwalela ngokucacileyo ukugcinwa efrijini nokwenziwa umkhenkce, ifuna iphakheji yokuqala ukuze kukhuselwe ekukhanyeni, kwaye iyalela ukuba kulahlwe okungasetyenziswanga kwizikhongozeli zayo zedosi enye. Le miyalelo yeyelo yeza. Ayiyomiyalelo yokugcina okanye yokuphinda kusetyenziswe ibhotile enesixhobo sokukhupha amathontsi ethengiswa ezivenkileni.

Ngoko ke, “okubanda ngakumbi kumele kube ngcono” ngumgaqo ongathembekanga. Ukuba imveliso iba ngumkhenkce xa ithunyelwa okanye ibhotile ihlala iiyure kwisithuthi esishushu, bhala oko kwenzekileyo uze ubuze umvelisi ukuba imveliso isamkelekile na. Ukuyibuyisela kubushushu obucetyiswayo akuqinisekisi ukuba ukuvezwa kwezo meko bekungenabungozi.

Umgubo ufuna imiyalelo yawo

Ukugcinwa komgubo akufani nje nokugcinwa kwesinyibiliko esikhutshwe amanzi. Funda iphakheji nephepha ledatha yokhuseleko lento oyinikiweyo. Imiyalelo yemveliso yomgubo kaBlupreme ifuna iphakheji evalwe ngokuqinileyo, iimeko ezomileyo nokukhuselwa ekukhanyeni.

Njengomnye umzekelo ongqamene nemveliso ethile, imiyalelo kaMerck yemethylene blue yeCertistain ichaza i-5–30 °C yaloo nto yomileyo isetyenziswa kwimicroscope. Ivumela ukuba okungaphakathi okunikezelweyo kusetyenziswe de kufike umhla wokuphelelwa ochaziweyo emva kokuvulwa okokuqala, ukuba ezo meko ziyagcinwa kwaye ibhotile ihlala ivalwe ngokuqinileyo. Oku akumiselanga umhla ofanayo kwisinyibiliko sokufaka umbala elebhu esilungiselelwe ngalo mgubo.

Ngaphambi kokuthenga ipakethi enkulu, jonga ukuba ungayisebenzisa na ngaphakathi kwexesha elibhalwe kumaxwebhu ayo kwaye uyigcine ivaliwe phakathi kwamaxesha okusetyenziswa. Isikhokelo sokuthenga umgubo sichaza imiba yodidi, ubukhulu bepakethi namaxwebhu.

Uluhlu lokuhlola ukugcinwa ngokusekelwe kwileyibhile

Sebenzisa le theyibhile isikhongozeli noxwebhu lwebhetshi oluhambelana naso ziphambi kwakho. Khuphela eyona miyalelo kwikholam yokugqibela. Indawo engenanto ibonisa umbuzo ekufuneka uphendulwe, hayi imeko ekufuneka uyiqashele.

Into ehlolwayo	Isinyibiliko esilungiselelweyo	Umgubo onikezelweyo	Ingxelo yale mveliso
Ukuchongwa	Eyona mveliso, ukuxinana, injongo yokusetyenziswa	Eyona mveliso, udidi, imo enikezelwe ngayo	Imveliso: ______
Ibhetshi	Tshatisa ibhotile namaxwebhu	Tshatisa ipakethi nesatifikethi	Ibhetshi: ______
Uhlobo lomhla	Umhla wokuphelelwa/ocetyiswayo wokusetyenziswa kunye nawo nawuphi na umhla wokunikezelwa ekhemesti	Umhla wokuphelelwa okanye wokuphinda kuvavanywe, hayi nje umhla wokukhutshwa	Uhlobo/umhla: ______
Ubushushu	Khuphela uluhlu lobushushu nomyalelo wokwenziwa umkhenkce/wokugcinwa efrijini	Khuphela uluhlu lobushushu olungqamene nomgubo	Iimeko: ______
Ukukhanya nokufuma	Khuphela iimfuno zokukhuselwa ekukhanyeni	Khuphela iimfuno zokukhuselwa ekukhanyeni nezokugcinwa kwindawo eyomileyo	Indawo: ______
Ukuvalwa	Ibhotile yokuqala, isiciko, nayo nayiphi na ibhokisi efunekayo	Ipakethi yokuqala nokuphinda ivalwe ngokufanelekileyo	Ukuvalwa kuhloliwe: ______
Emva kokuvulwa	Bhala umhla wokuvulwa nomda ochaziweyo wokusetyenziswa emva kokuvulwa	Jonga ukuba ukuvula kuyalitshintsha na ixesha elichaziweyo	Ivuliwe/umda: ______
Ixutyiwe namanzi okanye ilungisiwe	Fumana imiyalelo yomxube otshintshiweyo	Musa ukusebenzisa imihla yomgubo kwisinyibiliko esitsha	Uxwebhu olusebenzayo: ______
Isiganeko sokugcinwa	Bhala ubushushu, ukuba ngumkhenkce, ukuvuza okanye inkxalabo yokungcoliseka	Bhala ukufuma, iphakheji eyonakeleyo okanye ukuphambuka kwiimeko zokugcina	Isiganeko/inyathelo: ______

Kutheni isikhongozeli nokukhuselwa ekukhanyeni kubalulekile

Gcina ileyibhile nepakheji yokuqala ngaphandle kokuba imiyalelo yemveliso ifuna ngenye indlela. Ibhotile esetyenziswa endaweni yeyokuqala inokwahluka ekukhuseleni ekukhanyeni, ekuvalweni nasekuhambelaneni kwezinto eyenziwe ngazo nemveliso. Ingxoxo yeFDA yovavanyo lokuzinza ichonga umahluko kwindlela eyenziwe ngayo imveliso nakwinkqubo yesikhongozeli nesivalo njengezinto ezichaphazela ukumiselwa kwemihla yemveliso.

Ibhotile embala ubumdaka obutyheli ayenzi ukukhanya kwelanga okuthe ngqo kwamkeleke. Beka imveliso kwindawo ehlangabezana nemiyalelo yayo nephepha ukuvezwa kubushushu obunokulindeleka. Kwibhotile evuliweyo enesixhobo sokukhupha amathontsi, kuphephe ukusichukumisa ezandleni, emlonyeni okanye kumphezulu osebenzela kuwo; yivale ngokukhawuleza. Musa ukucinga ukuba umbala wedayi uqinisekisa umgangatho wayo ngokuphathelele iintsholongwane. Ingxoxo yobugcisa yokukhanya, i-pH, izinyibilikisi nokuzinza kwemethylene blue icacisa ukuba kutheni imifuniselo nemixube ifuna iimeko zayo ezithile.

Imihla engekhoyo okanye inkangeleko etshintshileyo

Ukuba awufumani mhla, cela umboneleli ukuba achonge uxwebhu lwebhetshi olusebenzayo aze acacise ukuba imveliso inomhlа wokuphelelwa, umhla wokuphinda kuvavanywe okanye omnye umda wokusetyenziswa obhalwe kumaxwebhu. Buza ngokwahlukeneyo ngokusetyenziswa emva kokuvulwa. Ingcaciso yenkxaso yeemveliso kaSigma-Aldrich ichaza ngokucacileyo ukuba ukungabikho komhla wokuphelelwa okanye wokuphinda kuvavanywe kunokubonisa ukungabikho kwedatha efanelekileyo eyaneleyo yokuzinza ukuze kumiselwe ixesha lokuhlala ilungele ukusetyenziswa. Ulwazi olungekhoyo alubobungqina bokuzinza okungapheliyo.

Ukuba mfiliba okungalindelekanga, amasuntswana, ukuvuza okanye itywina elonakeleyo kufuna ukuba uyeke ukusebenzisa imveliso uze ulandele imiyalelo yayo. Ibhotile ekhangeleka iqhelekile ayiluphumeleli ngaloo nto uvavanyo lokuphelelwa. Kwelinye icala, ukuba mnyama nje kunokubonisa indlela isinyibiliko esixinene kakhulu esifunxa ngayo ukukhanya. Funda ngezinyibiliko eziluhlaza, ezimfiliba okanye eziphantse zibe mnyama kunye nezinto eziqina ziphume kwisinyibiliko neekristale ngaphambi kokuthi utshintsho lwenkangeleko lubangelwe yinto enye. Musa ukufudumeza, ukuhluza okanye ukuphinda uxube imveliso ethandabuzekayo ukuze iphinde ibonakale yamkelekile.