Inqaku
Imethylene blue ekunciphiseni iintsholongwane ezibangela izifo kwiplasma: indlela inkqubo esebenza ngayo
Imethylene blue inokunceda ekunciphiseni iintsholongwane ezibangela izifo kwiplasma enikelweyo xa iyinxalenye yenkqubo yokulungisa elawulwayo. Olu nyango lwenzeka ngaphandle komzimba, ngaphambi kokuba iplasma ikhululwe ukuba isetyenziselwe utofelo-gazi. Ludibanisa izixhobo ezichaziweyo, ukuchanabeka kwidayi, ukukhanyisa, ukuhluza, nokuhlolwa kwecandelo legazi eliphumayo.
Lo mahluko umisela intsingiselo yobungqina. Uphononongo olubonisa ukuba inkqubo yokulungisa inciphisa intsholongwane ekwaziyo ukusulela kwingxowa yeplasma luqinisekisa isiphumo saloo mathiriyeli naloo nkqubo. Aluqinisekisi ukuba ukuthatha imethylene blue kunyanga usulelo emntwini.
Umbuzo obalulekileyo ngokwezonyango nawo unamacala amabini: inkqubo inciphisa iintsholongwane ezibangela izifo kangakanani, yaye umsebenzi oluncedo weplasma usala ungakanani?
Qala ngeplasma, kungekhona ngemveliso yegazi engachazwanga
Iplasma licandelo legazi elilulwelo elineeproteni, kuquka izinto ezinceda igazi lijiye. Akufanele kucingelwe ukuba inkqubo yokulungisa iplasma ifanelekile kwigazi elipheleleyo, kumxube oxineneyo weeseli ezibomvu, okanye kumacandelo aneeplatelet. Ezi mathiriyeli zahlukile ngokwakheka nangemisebenzi ekufuneka igcinwe.
Njengomzekelo ocacileyo, inkqubo yeTHERAFLEX MB-Plasma yakwaMacopharma inyanga iiyunithi zeplasma ezivela kumnikeli omnye. Ulandelelwano lwayo olubhaliweyo luquka ukunciphisa iiseli, ukongeza imethylene blue, ukukhanyisa, ukususa idayi nokugcina. Umenzi uchaza ulungiselelo olwahlukileyo lweplasma ethathwe kwigazi elipheleleyo nolweengqokelela ezinkulu ezifunyenwe ngeapheresis. Ngoko ke imathiriyeli ekuqalwa ngayo neseti yezixhobo ezisetyenziswa kube kanye zibalulekile ngaphambi kokuba ukukhanyisa kuqale.
“Iplasma yemethylene blue” yinkcazelo yecandelo elilungisiweyo, ayizizo iinkcukacha ezipheleleyo zemigangatho yalo. Uthelekiso oluluncedo luchaza inkqubo, ulungiselelo, umthombo weplasma nohlelo lwemiyalelo yokusebenza.
Landela imathiriyeli kuwo onke amanqanaba okulungisa novavanyo
Umfanekiso ongentla wahlula amanqanaba. Le theyibhile ihamba nawo ichaza igalelo lenqanaba ngalinye noko lingenako ukukuqinisekisa lodwa.
| Inqanaba lokulungisa | Injongo ephambili | Umbuzo owahlukileyo ekufuneka uhlolwe |
|---|---|---|
| Ukunciphisa iiseli ngaphambi kokuchanabeka | Ukususa iiseli zegazi ezishiyekileyo namaqela azo aqokeleleneyo kwiplasma | Ingaba imathiriyeli ekuqalwe ngayo ibifanelekile kolu lungiselelo? |
| Ukuchanabeka kwidayi | Ukudibanisa into evuselelwa kukukhanya nemathiriyeli eza kunyangwa | Ingaba ukuchanabeka kufezekiswe phantsi kweemeko ezichaziweyo zenkqubo? |
| Ukukhanyisa okulawulwayo | Ukuqhuba ukuphendulana kweekhemikhali okubangelwa kukukhanya okunciphisa iintsholongwane ezichaphazelekayo | Ingaba iimeko zokukhanya nezobushushu eziqinisekisiweyo zifezekisiwe? |
| Ukususwa kwedayi nezinto eziveliswa kukukhanya | Ukunciphisa iikhemikhali zokulungisa ezishiyekileyo ngaphambi kokugcina | Ingaba inyathelo lokususa lizifezekisile iimfuno zenkqubo? |
| Ukuvavanywa nokukhululwa kwemveliso | Ukuvavanya iirekhodi zokulungisa nomgangatho wecandelo ngokweenkqubo zenkonzo yegazi | Ingaba icandelo eligqityiweyo liyawufezekisa umgangatho osebenzayo kulo? |
Le theyibhile sisikhokelo sokufunda inkqubo eqinisekisiweyo, ayithathi indawo yemiyalelo yokusebenza. Ngokukodwa, isihluzi sokususa nomthombo wokukhanya zenza imisebenzi eyahlukileyo. IMacopharma ichaza iBlueflex njengenciphisa imethylene blue nezinto eziphuma kuyo, kuquka iikhompawundi zeazure nethionine. Ibango lokususwa kweekhemikhali kufuneka liqondwe njengokuncipha okulinganisiweyo, kungekhona njengesithembiso sokuba akukho molekyuli ishiyekileyo.
Kutheni umthombo wokukhanya uyinxalenye yonyango
Imethylene blue iyavuseleleka kukukhanya. Ukukhanyisa kunokubangela umonakalo weekhemikhali kwizinto eziphilayo ekujoliswe kuzo, nto leyo ebangele ukuba le ndibaniselwano iphandwe ngenjongo yokwenza iintsholongwane zingakwazi ukusulela. Kuphononongo lukaMohr noogxa bakhe ngeedayi zephenothiazine nokukhanya, ukutshintsha umthombo wokukhanyisa kwatshintsha kokubini ukungakwazi kweentsholongwane ukusulela nomonakalo kwiiproteni zeplasma.
Oku kuthintela indlela emfutshane eqhelekileyo: ukuphatha igama ledayi ngokungathi lichaza lonke unyango. Isibane, ingxowa, indlela edlula kuyo ukukhanya, okanye imathiriyeli eyahlukileyo inokutshintsha ukuchanabeka. Isikhokelo esibanzi sendlela olufundwa ngayo unyango lwemethylene blue oluvuselelwa kukukhanya sichaza ukuba kutheni ukwakheka komxube nokukhanyisa kufuneka kuchazwe kunye.
Ubushushu bunika umzekelo oluncedo ngokukhethekileyo. Uphononongo luka2018 lokulungisa ngeTHERAFLEX kumaqondo obushushu ahlukeneyo lwathelekisa iplasma ekufutshane namaqondo ayi5°C, 22°C, no30°C. Kwelona qondo lobushushu lisezantsi, abaphandi babona ukuba ithebhulethi yayinyibilika kakubi nokuba iintsholongwane ezintathu ezazifundwa zazisenziwa zingakwazi ukusulela ngomlinganiselo ophantsi. Amaqondo obushushu aphezulu andisa utshintsho kwixesha lokujiyisa nokulahleka komsebenzi wezinto ezinceda igazi lijiye.
Ezo ziphumo azibeki luluhlu lutsha lweemeko zokusebenza. Zichaza ukuba kutheni inkonzo yegazi kufuneka isebenzise iimeko ezichaziweyo: ukuphucula esinye isiphumo ngokutshintsha umlinganiselo othile kunokonakalisa esinye.
Leliphi icandelo lenkqubo elanciphisa intsholongwane?
Ukuncipha kweentsholongwane ezibangela izifo okulinganiswe emva kwayo yonke inkqubo akunakubalwa ngokuzenzekelayo njengomsebenzi wedayi evuselelwe kukukhanya kuphela.
Kuphononongo lovavanyo luka2015 olusebenzisa iplasma engcoliswe ngabom, abaphandi bathatha iisampulu zemathiriyeli emva kwamanqanaba ngamanye okulungisa. Ukuhluzwa kweeseli kwaba negalelo kwinxalenye enkulu yokuncipha kweebhaktheriya. Kwibhaktheriya encinci iBrevundimonas diminuta, elo nyathelo lokuqala lafezekisa ukuncipha nge-1.7-log; unyango lokukhanya nokuhluzwa kwedayi okwalandelayo kwazisa ukuncipha okupheleleyo ubuncinane kwi-3.7 logs, ngaphantsi komda wokufumanisa wovavanyo.
Esi sisiphumo senkqubo edityanisiweyo. Asibonisi ukuba isisombululo esiqhelekileyo semethylene blue sivelisa ukuncipha okufanayo kweebhaktheriya. Lo mahluko ukwasebenza xa kufundwa uphando olubanzi ngemethylene blue ngokuchasene neentsholongwane.
Ukuncipha ngokwelog kuchaza umlinganiselo phakathi kwemathiriyeli eyalinganiswa ekuqaleni neshiyekileyo ekwaziyo ukusulela. Ukuncipha ngeelog ezintathu kukuncipha ngokuphindwe kaliwaka. “Ngaphantsi komda wokufumanisa” kuthetha ukuba uvavanyo alukwazanga ukulinganisa okushiyekileyo phantsi kweemeko zalo zokuvavanya. Ayibobungqina bokungabikho ngokupheleleyo kweentsholongwane okanye bokusebenza kuzo zonke izinto eziphilayo ezingavavanywanga. Uhlobo lwentsholongwane, ubungakanani bayo ekuqaleni, ukwakheka kwesampulu, novakalelo lovavanyo zihlala ziyinxalenye eyimfuneko yebango.
Gcina umsebenzi eza kusetyenziselwa wona iplasma
Inkqubo inokunciphisa amandla okubangela usulelo ngoxa itshintsha iiproteni ezibalulekileyo ngokwezonyango. Ngoko ke ukulinganisa ukuncipha kweentsholongwane ezibangela izifo kuphela kushiya uvavanyo lungaphelelanga.
Kuthelekiso luka2014 lwemithombo yeplasma namaxesha okulungisa, umlinganiselo wefactor VIII ogciniweyo emva konyango lwemethylene blue wawusukela ku78% ukuya ku89% kuwo onke amaqela ophononongo. Lo mlinganiselo wawuxhomekeke kumthombo weplasma, yaye abaphandi bavavanya nefibrinogen. Le yimilinganiselo yaselebhu eyenziwe kwicandelo elilungisiweyo. Ayizizo iipesenti zokusebenza ngokwezonyango kwizigulana.
Qwalasela iiyunithi ezimbini zeplasma ezinamanqanaba ahlukeneyo ezi zinto ekuqaleni. Nokuba zigcina iqhezu elifanayo lomsebenzi ngexesha lonyango, amanqanaba azo okugqibela aya kwahluka. Ngokuchaseneyo, ipesenti ephezulu egciniweyo ayithethi ukuba ukuxinana kokugqibela kuphezulu. Funda kokubini imilinganiselo yangaphambi nasemva, kunye nomgangatho osebenzayo wokukhulula icandelo.
Iziphumo kwizigulana zifuna olunye uhlobo lobungqina. Uphononongo lomgangatho wecandelo alunako, lulodwa, ukuqinisekisa ukulingana kolawulo lokopha, kweemfuno zotofelo-gazi, okanye kwemilinganiselo yeziganeko ezingathandekiyo kwiqela elithile lezigulana.
Gcina ubungqina bokulungisa bahlukene namabango onyango
Ingxowa yeplasma yenza ukuchanabeka okulawulwayo kwenzeke: inomthamo, imilo, ulandelelwano lokulungisa, kunye nesiphumo esinokulinganiswa ezichazwe ngokucacileyo. Umntu unezicubu, ukujikeleza kwegazi, iinkqubo zokuguqula izinto emzimbeni, neendawo zosulelo ezingaveliswanga kolu vavanyo.
Ngenxa yoko, amabango onyango lweHIV xa ethelekiswa nobungqina bokwenza iintsholongwane kwiplasma zingakwazi ukusulela kunye nobungqina bemethylene blue ngeCOVID-19 neCOVID ehlala ixesha elide zifuna uvavanyo lwazo lwezonyango. Akukho namnye kule mibuzo uphendulwa ngokubonisa ukuncipha kwamandla okubangela usulelo kwimathiriyeli enikelweyo.
Xa isetyenziselwa inkonzo yegazi, vavanya elona candelo lichanekileyo nenkqubo evunyelweyo ekuhlaleni, ukuqinisekiswa kwayo kwintsholongwane nganye, umsebenzi weeproteni ogciniweyo, iikhemikhali ezishiyekileyo, nolawulo lokukhulula icandelo. Ukufumaneka kwemveliso kuyahluka ngokwamazwe, njengoko umenzi esitsho. Ukusetyenziswa kwayo ngaphambili okanye uvavanyo oluyimpumelelo akuqinisekisi ukuba ngoku ivunyelwe kuyo yonke indawo. Iyunithi eluncedo yobungqina yinkqubo epheleleyo eqinisekisiweyo esetyenziswa kwicandelo leplasma elichaziweyo.