Igikoresho gihindura konsantrasiyo y'ubururu bwa metilene: % muri mg/mL

Umushongi w'ubururu bwa metilene wa 1% w/v urimo 10 mg/mL. Ikirango cyanditseho gusa “1%” gisiga ikibazo cy'ingenzi kidasubijwe: ni garama imwe muri mililitiro 100 z'umushongi, cyangwa garama imwe muri garama 100? Iya mbere ihindurwa ako kanya muri mg/mL. Iya kabiri isaba kumenya ubucucike bw'umushongi.

Fungura igikoresho gihindura konsantrasiyo kugira ngo winjize uburyo igipimo cyo ku kirango gisobanuwe, konsantrasiyo, na volime yapimwe. Iki gikoresho gikorera muri mushakisha yawe kandi gishobora kubikwa kugira ngo gikoreshwe nta murandasi. Kibara mase y'ikinyabutabire n'ibipimo bingana na yo mu zindi ngero. Ntigihitamo igipimo umuntu ahabwa, uburyo ahabwamo ikinyabutabire, cyangwa gahunda yo kuvurwa. Ibyo bibazo bisaba irindi suzuma, risobanurwa mu ngingo ivuga impamvu konsantrasiyo atari inama y'igipimo cyo guhabwa.

Soma umugabanya mbere y'umubare

Konsantrasiyo isobanura ingano y'ikigize imvange ugereranyije n'ingano runaka y'iyo mvange. Umugabanya ni wo ugena icyo umubare usobanura. NIST itandukanya konsantrasiyo ya mase na konsantrasiyo y'ingano y'ikinyabutabire: mg/mL isobanura mase kuri volime, naho mol/L igasobanura ingano y'ikinyabutabire kuri volime. Ibyo ni ibipimo bitandukanye.

Kuri iki gikoresho, koresha ibi bisobanuro by'amakuru winjiza:

Amakuru winjiza	Icyo asobanura	Icyo winjiza
Ijanisha w/v	Garama z'ikigize umushongi cyavuzwe muri 100 mL z'umushongi wa nyuma	Injiza `1` kuri 1%, aho kwinjiza `0.01`
Ijanisha w/w	Garama z'ikigize umushongi cyavuzwe muri 100 g z'umushongi wose	Injiza ijanisha n'ubucucike bw'umushongi muri g/mL
mg/mL	Miligarama z'ikigize umushongi cyavuzwe muri mililitiro imwe y'umushongi	Injiza konsantrasiyo ya mase yatangajwe uko iri
Volime yapimwe	Volime y'umushongi uri gusuzumwa	Injiza mL; 100 µL ni 0.1 mL
Volime y'igitonyanga yapimwe	Impuzandengo ya volime yapimwe itangwa n'igitonyanga kimwe	Injiza mL/drop gusa iyo izwi kuri ayo mazi n'igikoresho kiyatanga

Ibisobanuro by'imishongi bya EMD Chemicals bitandukanya mu buryo bweruye w/v, w/w, na v/v. Iki gikoresho cyo kubara nticyakira ijanisha v/v, risobanura isano hagati ya volime kandi ridashobora guhindurwa muri mase y'ikinyabutabire cyashongeshejwe hakoreshejwe ijanisha ryonyine.

Hindura ijanisha w/v urishyire muri mg/mL

Ku ijanisha ryatangajwe p rishingiye kuri w/v:

Konsantrasiyo muri mg/mL = p × 10

Umubare wa 10 uturuka ku guhindura ingero: garama p muri 100 mL zingana na miligarama p × 1,000 zigabanyijwe na 100 mL. Kugira ngo uhindure mu cyerekezo kinyuranye, gabanya mg/mL na 10.

Konsantrasiyo yatangajwe	Konsantrasiyo ya mase bingana	Mase iri muri 0.25 mL
0.1% w/v	1 mg/mL	0.25 mg
0.5% w/v	5 mg/mL	1.25 mg
1% w/v	10 mg/mL	2.5 mg
2% w/v	20 mg/mL	5 mg

Izi ni ingero z'imibare, si ibipimo bisabwa guhabwa umuntu. Zirikana ko gukuba konsantrasiyo kabiri bikuba kabiri mase iri muri volime imwe. Amabwiriza ya volime ntashobora kwimurirwa hagati y'imishongi ifite konsantrasiyo zitandukanye utabanje kugenzura konsantrasiyo.

“Umushongi wa nyuma” na byo ni ingenzi. Igipimo cya garama imwe muri 100 mL gisobanura volime yose nyuma yo gushonga. Ntibisobanura kongera garama imwe muri 100 mL z'amazi yamaze gupimwa. Mu gutegura umushongi, volime ya nyuma ni yo ngano fatizo.

Hindura ijanisha w/w ukoresheje ubucucike bw'umushongi

Ku ijanisha p rishingiye kuri w/w n'ubucucike bw'umushongi ρ muri g/mL:

Konsantrasiyo muri mg/mL = p × 10 × ρ

Dufate umushongi w'urugero wa 1% w/w ufite ubucucike bwanditswe bwa 1.05 g/mL. Mililitiro imwe ipima 1.05 g. Ijanisha rimwe ry'iyo mase ni ikinyabutabire cyashongeshejwe cyavuzwe: 0.0105 g, cyangwa 10.5 mg. Bityo konsantrasiyo ya mase yawo ni 10.5 mg/mL, ihwanye na 1.05% w/v.

Ubucucike bwo muri urwo rugero bwatoranyijwe gusa kugira ngo busobanure imibare. Si agaciro gapimwe ku gicuruzwa cya Blupreme. Injiza ubucucike bw'umushongi nyirizina warangije gutegurwa, ku bushyuhe bireba. Ubucucike bw'amazi cyangwa ubw'ifu yumye y'ubururu bwa metilene ni ikindi gipimo. Iyo ubucucike butaboneka, igikoresho gisiga ibarura rya w/w ritarakemuka aho kwinjizamo 1 g/mL nta kubivuga.

Mu ibarura rinyuranye, ijanisha w/w ringana na mg/mL bigabanyijwe na 10 × ubucucike muri g/mL. Ibi bisobanura impamvu w/w na w/v bishobora kugira imibare isa mu mvange zimwe z'amazi zirimo ikinyabutabire gike, ariko ibisobanuro byabyo ntibishobore gusimburana.

Bara mase iri muri volime yapimwe

Iyo konsantrasiyo imaze kugaragazwa muri mg/mL:

Mase muri mg = konsantrasiyo muri mg/mL × volime muri mL

Umugabane wa 0.25 mL upimwe muri laboratwari uvanywe mu mushongi fatizo wa 5 mg/mL urimo 1.25 mg z'ikigize umushongi cyavuzwe mu bisobanuro bya konsantrasiyo. “Umugabane” hano bisobanura igice cy'umushongi cyapimwe. Ibarura rifata ko uwo mugabane uhagarariye umushongi wavuzwe ufite ibigize byakwirakwiye kimwe hose.

Igikoresho cyo gupima ni cyo kigena niba koko 0.25 mL zatanzwe. Eppendorf isobanura uko imiterere y'amazi n'ubushyuhe bigira ingaruka ku gukoresha pipeti ifite umusego w'umwuka. Imibare ikozwe neza ntishobora gukosora igikoresho cyo gupima kidakwiriye, akabumbe k'umwuka, cyangwa igipimo cyasomwe nabi. Igisubizo cyerekanwe gifite imibare itandatu y'ingenzi ni umusaruro w'ibarura, si ukwemeza ko igipimo ari nyacyo ku rwego rw'imibare itandatu.

Impamvu nta mubare w'ibitonyanga ushyirwaho mbere

Ikibazo kigira kiti “Ni ibitonyanga bingahe birimo 1 mg?” gisaba kumenya konsantrasiyo na volime yapimwe y'igitonyanga kimwe. “Igitonyanga kimwe” cyangwa uko igikoresho gitanga ibitonyanga gisa ntibitanga iyo volime.

Niba impuzandengo yapimwe ya volime y'igitonyanga ari d mL/drop:

Mase muri buri gitonyanga = konsantrasiyo × d

Ku ipimwa rya 0.04 mL/drop rifashwe nk'urugero gusa n'umushongi wa 10 mg/mL, impuzandengo ya mase ni 0.4 mg/drop. Mu mibare, miligarama imwe ihwanye na 2.5 by'ibyo bitonyanga by'impuzandengo. Icyo gisubizo kirimo umugabane ntigisobanura ko igice cy'igitonyanga gishobora gutangwa mu buryo bwizewe, kandi si amabwiriza yo guhabwa ikinyabutabire.

Ubushakashatsi bwa NIST ku bipimo by'ibitonyanga bugena mase na volime by'igitonyanga gitanzwe binyuze mu gupima. Ibisubizo byayo ku bikoresho bitanga ibitonyanga mu buryo bunonosoye ntibishobora gufatwa nk'ibireba igikoresho gisanzwe gitanga ibitonyanga ku mukoresha. Koresha amakuru y'ipimwa yerekeye amazi nyirizina, igikoresho kiyatanga, n'imiterere y'imikoreshereze. Siga umwanya w'amakuru atari ngombwa ubusa iyo ayo makuru adahari; igikoresho kizakomeza kubara mase gishingiye kuri volime yapimwe.

Gumana ishingiro ry'ikinyabutabire ryanditse ku kirango

Igikoresho gikoresha konsantrasiyo yinjijwe uko iri mu ibarura. Ntigikosora imibare hakurikijwe igipimo cy'ubuziranenge bw'ifu, ntigihindura hagati y'ishingiro ry'ikinyabutabire kirimo amazi mu miterere yacyo n'iritayafite, ntikigaragaza umwanda urimo, kandi nticyemeza ko umushongi ukwiranye n'imikoreshereze runaka. Andukura ibisobanuro byuzuye by'ikinyabutabire bivuye mu nyandiko y'inkomoko igihe wandika igisubizo. Imfashanyigisho kuri konsantrasiyo y'umushongi w'ubururu bwa metilene isobanura uko wagereranya ibisobanuro bya konsantrasiyo ku bicuruzwa bitandukanye.

Mbere yo gukoresha igisubizo, andika ibi bintu bitatu hamwe: konsantrasiyo y'umwimerere n'ishingiro ryayo, volime yapimwe, na mase yabazwe. Ongeraho ubucucike cyangwa ipimwa ry'ibitonyanga iyo kimwe muri byo cyakoreshejwe. Iyo nyandiko ituma ibarura rishobora gusubirwamo kandi igaragaza ibyafashwe nk'ukuri umubare wonyine utagaragaza.